Bst 2.0 DNA聚合酶来源于Bacillus Stearothermophilus DNA Polymerase I，通过基因工程手段去除了其5’-3’外切酶活性，而保留了5’-3’聚合酶活性。该酶具有很强的链置换能力。与野生型Bst DNA聚合酶（大片段）相比，该酶在扩增产量、耐盐性和热稳定性等方面均有大幅提高，同时，Bst 2.0 DNA聚合酶可以使用部分(50%)dUTP代替dTTP作为底物进行扩增(Bst 大片段无此活性)。

单位定义

1U是指在65°C条件下，30分钟内催化10nmol dNTP的掺入反应，成为酸不溶性物质所需的酶量。

酶储存液

10mM Tris-HCl, 150mM KCl, 2mM DTT, 0.1mM EDTA, 50%Glycerol, pH7.5。

热失活

85°C，5min。

性能展示

Bst 2.0 DNA模版扩增能力展示
20ul标准体系下采用0U（泳道1）、2U（泳道2）、4U（泳道3）、8U（泳道4）进行LAMP扩增。

应用LAMP产品文献

1. Rapid Visual Detection of Elite Erect Panicle Dense and Erect Panicle 1 Allele for Marker-Assisted Improvement in Rice (Oryza sativa L.) Using the Loop-Mediated Isothermal Amplification Method. Curr Issues Mol Biol. 2024 Jan 4;46(1):498–512.
2. Rapid Visual Detection of High Nitrogen-Use Efficiency Gene OsGRF4 in Rice (Oryza sativa L.) Using Loop-Mediated Isothermal Amplification Method. Genes (Basel). 2023 Sep 23;14(10):1850.
3. Rapid and visual identification of HIV-1 using reverse transcription loop-mediated isothermal amplification integrated with a gold nanoparticle-based lateral flow assay platform. Front Microbiol. 2023 Jul 12;14:1230533.
4. Rapid Detection of Streptococcus mutans Using an Integrated Microfluidic System with Loop-Mediated Isothermal Amplification. J Microbiol Biotechnol. 2023 May 19;33(8):1101–1110.
5. Rapid point-of-care detection of BK virus in urine by an HFman probe-based loop-mediated isothermal amplification assay and a finger-driven microfluidic chip.PeerJ. 2023 Mar 8;11:e14943.
6. Rapid detection of monkeypox virus and monkey B virus by a multiplex loop-mediated isothermal amplification assay. J Infect. 2023 Feb 13;86(4):e114–e116.
7. SMART: A Swing-Assisted Multiplexed Analyzer for Point-of-Care Respiratory Tract Infection Testing. Biosensors (Basel). 2023 Feb 4;13(2):228.
8. An HFman Probe-Based Multiplex Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Simultaneous Detection of Hantaan and Seoul Viruses. Diagnostics (Basel). 2022 Aug 10;12(8):1925.
9. Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method. Biosensors (Basel). 2022 Apr 3;12(4):213.
10.Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe.ACS Sens. 2022 Feb 22;7(3):730–739.
11.Deep‐dLAMP: Deep Learning‐Enabled Polydisperse Emulsion‐Based Digital Loop‐Mediated Isothermal Amplification. Adv Sci (Weinh). 2022 Jan 24;9(9):2105450.
12.Development of a TaqMan loop-mediated isothermal amplification assay for the rapid detection of pigeon paramyxovirus type 1. Arch Virol. 2021 Mar 23;166(6):1599–1605.
13.Rapid, visual, label-based biosensor platform for identification of hepatitis C virus in clinical applications. BMC Microbiol. 2024 Feb 28;24:68.
14.A novel extraction-free dual HiFi-LAMP assay for detection of methicillin-sensitive and methicillin-resistant Staphylococcus aureus.Microbiol Spectr. 2024 Feb 20;12(4):e04133-23.
15.A duplex HiFi-LAMP assay for screening of two novel human circoviruses HCirV-1 and HCirV-2. BMC Infect Dis. 2024 Dec 5;24:1388.
16.Point-of-care test of blood Plasmodium RNA within a Pasteur pipette using a novel isothermal amplification without nucleic acid purification. Infect Dis Poverty. 2024 Oct 31;13:80.
17.Rapid detection of human influenza A viruses by HFman probe-based loop-mediated isothermal amplification assays. Heliyon. 2023 Oct 25;9(11):e21591.